Seth G.S. Medical college,K.E.M. Hospital and N.Wadia Hospital, India
Title: New Laparoscopic vaginoplasty developing normal vagina, Identification of the progenitor cell and Genetic decoding of MRKH syndrome.
Dr. Pravin Mhatre is the Scientific Director of Kedar Hopital which has already made its mark as one of the reputed hospitals for gynecology services in India. Dr. Mhatre is a consultant to the Breach Candy Hospital, N. Wadia Hospital in Mumbai. He has done pioneering work in the field of Ovarian Transplant and has been credited with world's first successful "Ovarian Transplant".He has developed a new Vaginoplasty technique resulting in formation of normal Vagina and identification of Stem cell. He has performed more than 100 cases of Vaginoplasty. Dr. Mhatre has served as Honorary professor emeritus of Gyn/Obst at Seth G.S. Medical college / N. Wadia Hospital for 30 years.For more than 8 years Dr. Mhatre has conducted many rural tubal ligation camps all over India. For this honorary contribution in women's heath care his team has received Presidents Medal from government of India. Dr. Mhatre has delivered guest lectures in many international and national congresses and has been an invited Professor at Oxford University (UK), University of Georgia (Atlanta USA) & Colombo University. Dr. Mhatre has been honored as first Gyn.Transplant surgeon by government. He has been Interviewed on BBC London / Asia live on 7th May 2002 and 18th December 2002 for the worlds first successful Ovarian Transplant. BBC World on 24th December 2002 declared this Ovarian Transplant as The significant medical achievement of the year 2002.He has started the First Ovarian Bank in 2005, currently in joint collaboration with NIRRH and TATA Hospital
Host of vaginoplasty techniques have been described. None has been successful in developing normal vagina. Modified laparoscopic peritoneal vaginoplasty (LPV) by Dr. Mhatre’s technique is performed in Mayer–Rokitansky–KuÌˆster–Hauser syndrome (MRKHS) culminating in normal vaginal development.These serial metaplastic changes converting peritoneum to normal vagina are documented and the progenitor cell responsible for this change is identified.
- This study aims to confirm normal development of neovagina by anatomical and functional parameters of histology, cytology, and ultrasonography (USG) with Doppler in LPV.
- To identify peritoneal progenitor cell by OCT4/SOX2 immuno-histochemistry markers.
- To demonstrate the metaplastic conversion of peritoneum to neovagina and the progenitor cell concentration, distribution pattern.
This is prospective experimental study, conducted at teaching hospital and private hospital. Seventy-five women of MRKHS underwent LPV followed by histology, cytology, pH, three / four dimensional USG along with Doppler blood flow of neovagina. Nine women underwent peritoneal biopsy for identification of progenitor cells with OCT4/SOX2 markers. One patient underwent serial biopsies for 4 weeks for histology and progenitor cell immunohistochemistry.Eighteen women of MRKH and fifteen controls were subjected for genetic workup.