Saurabh Kapoor
Asst. Embryologist in a fertility centre located in Jalandhar city (Punjab, India).
Biography:
Mr. Saurabh is working as an Asst. Embryologist in a fertility centre located in Jalandhar city (Punjab, India). I have done my masters in Clinical Microbiology. I have done hands on as well as theoretical course in Comprehensive Andrology and Male Infertility from Delhi Gynaecologist forum’s IVF- Infertility Committee, Delhi and Intensive hands on training in Clinical Embryology from Asia Pacific Institute of embryology, Mysore, India. Worked as a team member in many events of life science/biological science. Pursuing Ph.D in (Life Science).
Abstract:
The present study was carried out to isolate, identify and to find out the Antibiotic Sensitivity Pattern of Microorganisms by which we can improve the fertilization rate as well as pregnancy rates. The mentioned study was carried out in the ART laboratory from April 2018 –September 2018 to identify various bacteria, grown in the samples and in media used in the Embryology Laboratory. Rarely, bacterial contamination occurs in the IUI/IVF/ICSI programme because IVF/ICSI laboratory and OT is maintained with filtered air by using air filtration unit. In case of disturbance or any other technical problem in the air filtration unit there are chances of bacterial infection. Bacterial growth in the media occurs only due to improper storage/handling (whenever used during the specific type of procedure).The main source of contamination is not only the environment in which work is done, patient’s body fluids like follicular aspirates, semen, vaginal, cervical regions, collected oocytes and handling of Gamets are also the potential sources of bacterial contamination.
Total 450 samples were observed/examined. Out of 450 samples, total (42, 09.33%) samples were found as positive (bacterial growth) and (408, 90.66%) Negative or with no growth after 24 hours of incubation in bacteriological incubator. Total 5 bacterial genus were identified (n = 02, 40%) gram positive and (n = 03, 60%) gram negative bacteria. In gram positive bacteria Lactobacillus spp. (10, 23.80%) and Staphylococcus aureus (12, 28.57%) and gram negative bacteria E. coli (08,
19.04%), Klebsiella spp. (06, 14.28%), Pseudomonas spp. (06, 14.28%) were identified. The presence of bacterial contamination on catheter tips during embryo transfer is evidently limited and does not significantly affect the cycle outcomes. End point measures are not affected by commensal contamination due to presence of different types of antibiotics in medium.